marker-free相关论文
Comparative analysis of transgenic wheat plants obtained by particle bombardment-mediated transforma
Aworkable particle bombardment-mediated common wheat transformation system has been developed for generating transgenicw......
RNA silencing is a post-transcriptional genesilencing phenomenon induced by double-stranded RNA (dsRNA).In an attempt to......
Marker gene excision in transgenic Brassica napus via Agrobacterium-mediated Cre/lox transient expre
Oilseed rape (Brassica napus L.) is one of the most important oil crops in the world.However,study on marker-free transg......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Introducing antisense waxy gene into rice seeds reduces grain amylose contents using a safe transgen
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Introducing antisense waxy gene into rice seeds reduces grain amylose contents using a safe transgen
An agrobacterium tumefaciens-mediated co-tr- ansformation method was established and successfully used to transfer an an......
Viruses are very widespread in nature,and they cause severe diseases and yield losses in potato production.The transfer ......
香味是稻米食味品质的主要性状之一,为培育无抗性选择标记基因的优质香稻新品系,以携带有超双元载体OsBADH2-RNAi的农杆菌菌株EHA1......
目前无选择标记技术已经成为基因工程中安全转基因研究方面的一个重要内容,转基因植物中标记基因的去除,一方面有利于消除公众对转......
利用无标记基因表达载体开展转基因植物研究,是获得无标记基因转基因植物、保证转基因生物安全的手段之一。文章报道了一种无标记......
香味是稻米食味品质的主要性状之一,为培育无抗性选择标记基因的优质香稻新品系,以携带有超双元载体OSBADH2-RNAi的农杆菌菌株EHA1......
构建了无标记基因源自玉米矮花叶病的主要病原--甘蔗花叶病毒（sugarcane mosaic virus,SCMV）复制酶基因的反向重复序列表达载体,通过......
利用双农杆菌双质粒的共转化系统获得了无选择标记转pepc基因的水稻植株.采用两个农杆菌EHA105转化粳稻品种台粳9号幼胚诱导的胚性......
筛选标记基因在转基因植物应用中存在一定潜在安全风险,在转基因植物改良中如何合理消除该基因非常必要。转录因子PTF1具有改善植......
综述了近年来国内外在植物无选择标记转基因技术方面的发展和研究成果，包括共转化法、转座子法（Ac／Ds转座子系统）、位点特异性重组系统......
目前,几乎所有的植物遗传转化都要通过使用选择标记基因,如抗生素或除草剂抗性基因等来筛选转化子,虽然没有研究结果表明选择标记......
Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9
Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. Th......
以p1300(含潮霉素抗性标记基因)和p13W8(含反义蜡质基因)为供体DNA,通过根癌农杆菌(Agrobacterium tumefaciens)介导的方法,对水稻......
利用甘蓝型油菜种子特异性贮存蛋白基因cruciferin启动子及NOS终止子构建了无选择标记基因且含有反义结构的油酸脱饱和酶基因（几以）......
以含多毛番茄GGPS基因的表达载体pBI121-GGPS和无标记载体pBINMF-GUS为基础,利用载体pBINMF-GUS的T-DNA区无选择标记基因的特点,将......
试验旨在构建一个能够定点整合且无筛选标记基因的半乳糖苷酶基因LacS表达载体。本研究以pEGFP-N1质粒为原始框架,通过PCR及限制性......
转基因植物中选择标记的存在是影响转基因植物安全的重要因素之一。目前主要从两个方面来解决转基因植物中选择标记的安全问题,一......
Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic p
Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergenc......
转基因植物的安全性是基因工程改良作物的一个重要问题.构建含双边界序列(双T-DNA区)载体,将选择标记基因和目标外源基因分开在不......
Marker gene excision in transgenic Brassica napus via Agrobacterium-mediated Cre/lox transient expre
Oilseed rape(Brassica napus L.)is one of the most important oil crops in the world.However,study on marker-free transgen......
Genetic and agronomic traits stability of marker-free transgenic wheat plants generated from Agrobac
Genetically modified wheat has not been commercially utilized in agriculture largely due to regulatory hurdles associate......
